D. Collen Young Investigator Awards Lecture

8:00 am- Wednesday, Oct 19th
　Chair: Paul J Declerck and Mario Colucci
YIA-1 (PS5-02)--A Novel Thrombolytic Drug based on Thrombin Cleavable Microplasminogen Coupled to a Single-Chain Antibody Specific for Activated GPIIb/IIIa
　Thomas Bonnard, Zachary Tennant, Ruchi Kanojia, Karen Alt, Shweta Jagdale, Lok Soon Law, Sheena Rigby, Karlheinz Peter, Christoph Hagemeyer
YIA-2 (PS3-08)--Does the Plasminogen Activation System Contribute to Immunosuppression after Traumatic Brain Injury?
　Dominik F. Draxler, Maria Daglas, Gryselda Hanafi, Adam Galle, Amanda E Au, Magdalena Plebanski, Maithili Sashindranath, Robert L. Medcalf
YIA-3 (PS2-09)--Plasminogen Activator Inhibitor 1 Associates with the Activated Platelet Membrane via a Fibrin and αIIbβ3 Specific Mechanism
　Gael B Morrow, Nicola J Mutch, Claire S Whyte
YIA-4 (PS3-03)--The neuronal tPA contributes to the physiological corticogenesis by promoting NMDA receptors signaling in the glial radial fibers
　Nolwenn Pasquet, Mikaël Naveau, Sara Douceau, Flavie Lesept, Yannick Hommet, Laurent Lebouvier, Denis Vivien, Isabelle Bardou
YIA-5 (PS3-05)--Tissue plasminogen activator (tPA) null mice decrease dopaminergic loss and increase behavioral recovery in a murine PD model
　Daniel Torrente, Gerald P Schielke, Kagya A Amoako, Mark Warnock, Daniel A Lawrence

Oral Session 1---Plasminogen Activation System (Protein Structure & Function)

8:30 am- Tuesday, Oct 18th
　Chairs: Paul Y. Kim and Dirk Hendriks
OS1-01 (PS2-01)--Lysine 42, 43, 44 and Arginine 12 within the Activation Peptide of Thrombin Activable Fibrinolysis Inhibitor Contribute to its Activation by the Thrombin-Thrombomodulin Complex in a Cooperative Fashion
　Paul Y. Kim, Chengliang Wu, James C. Fredenburgh, Jeffrey I. Weitz, Ann Gils, Paul J. Declerck
OS1-02--A mutation of highly conserved glycine in strand 5B of plasminogen activator inhibitor 1 caused its deficiency due to self-polymerization, which was also observed in other serine proteinase inhibitors
　Takayuki Iwaki, Kotomi Nagahashi, Katsuhiro Takano, Katsue Suzuki-Inoue, Naohiro Kanayama, Kazuo Umemura, Tetsumei Urano
OS1-03 (PS2-02)--Carboxypeptidase U (CPU, CPB2 or TAFIa) generation during experimental embolic stroke in rat
　Dorien Leenaerts, Jean-Philippe Desilles, Joachim C Mertens, Stéphane Loyau, Martine Jandrot-Perrus, Mikael Mazighi, Dirk Hendriks
OS1-04 (PS2-06)--Structural studies of the effect of inhibitory antibody fragments on plasminogen activator inhibitor 1 (PAI-1)
　Machteld Sillen, Stephen D. Weeks, Xiaohua Zhou, Sergei V. Strelkov, Paul J. Declerck
OS1-05--Complement anaphylatoxins are regulated by both basic carboxypetidases in plasma (CPB2 and CPN)
　John Morser, Zhifei Shao, Toshihiko Nishimura, Qin Zhou, Lawrence Leung
OS1-06--Extracellular Proteostasis: Synergy Between the tPA System and Extracellular Chaperones?
　Mark R. Wilson, Patrick Constantinescu, Marie Ranson

Oral Session 2---Plasminogen Activation System (Cell Biology)

11:00 am- Tuesday, Oct 18th
　Chairs: Marie Ranson and Maria Patrizia Stoppelli
OS2-01--PAI-1 Expression Renders M2 Polarized Macrophages Proteolytically Quiescent
　Philipp J. Hohensinner, Johanna Baumgartner, Pavel Uhrin, Benjamin Ebenbauer, Barbara Thaler, Konstantin Doberer, Christoph Kaun, Stefan Stoijkovic, Michael Fischer, Gernot Schabbauer, Johann Wojta, Walter S. Speidl
OS2-02--PAI-1 affects cellular migration and proliferation in a uPAR/LRP1- dependent manner via β-catenin and ERK1/2
　Thomas Kietzmann, Tabughang Franklin Chi, Jan Kristian Jensen
OS2-03--A Retro-inverso Urokinase Receptor Derived peptide for the Treatment of Metastatic Sarcoma
　Maria V. Carriero, Katia Bifulco, Vincenzo Ingangi, Concetta Ragone, Susan Costantini, Giovanni Botti, Antonello Pessi, Gennaro Ciliberto
OS2-04--IGF-1R and uPAR are Essential to Stromal Fibroblast Activation and Mobilization in Early Brest Tumorigenesis
　Maria Patrizia Stoppelli, Anna De Vincenzo, Stefania Belli, Paola Franco, Mara Iavazzo, Paola Monaco, Ingram Idelson Iaccarino
OS2-05--Hypoxia increased invasion of primary human extravillous trophoblast by urokinase-type plasminogen activator system
　Taihei Tsunemi, Katsuhiko Naruse, Natsuki Koike, Aiko Shigemitsu, Hiroshi Kobayashi

Oral Session 3---Vascular Biology

2:30 pm- Tuesday, Oct 18th
　Chairs: Lianchun Wang and Satoshi Fujii
OS3-01--Urokinase system is an important participant in the communication between endothelial and mesenchymal cells during angiogenesis
　Irina Beloglazova, Victoria Stepanova, Ekaterina Zubkova, Natalia Koptelova, Konstantin Dergilev, Vsevolod Tkachuk, Yelena Parfyonova
OS3-02--Predominance of tip-cell like behavior in endothelial cells derived from human PAI-1 deficient iPS cells
　Hideto Sano, Makoto Otsu, Takayuki Iwaki, Kotomi Nagahashi, Hiroki Tanaka, Yuko Suzuki, Naohiro Kanayama, Tetsumei Urano
OS3-03--Heparan Sulfate is a Novel Regulator of Fibrinolysis
　Junlin Jiang, Jingwen Yue, Xiang Fan, Dzung T. Le, Mortimer Poncz, Jefferey Esko, Lianchun Wang
OS3-04--Facilitated exocytosis of tissue-type plasminogen activator by fluid shear stress depends on plasma membrane polarity of vascular endothelial cells in part
　Yuko Suzuki, Kazutoshi Hashizume, Hideto Sano, Hiroki Tanaka, Ritsuko Ohtani-Kaneko, Tetsumei Urano
OS3-05--The role of the HIF-1α/HuR pathway in increased expression of PAI-1 induced by sphingosine 1-phosphate in HepG2 cells rendered hypoxic
　Satoshi Fujii, Akimasa Sanagawa, Soichiro Iwaki, Moyoko Asai, Daisuke Sakakibara, Hiroaki Norimoto
OS3-06--Co-operation of TNFalpha and VEGF to regulate PAI-1 through synergistically upregulated p53
　Sushil Panta, Munekazu Yamakuchi, Toshiaki Shimizu, Kazunori Takenouchi, Yoko Oyama, Teruto Hashiguchi

Oral Session 4---Cell-Associated Modification of Fibrinolysis

4:30 pm- Tuesday, Oct 18th
　Chairs: Nicola J Mutch and Seiji Madoiwa
OS4-01 (PS2-07)--Proteolytic activity of circulating microparticles from ischemic stroke patients revealed by single particle laser-scanning confocal microscopy
　Sara Martinez De Lizarrondo, Aurélien Briens, Jérôme Parcq, Joan Montaner, Denis Vivien, Maxime Gauberti
OS4-02 (PS2-08)--Lipid rafts, αIIbβ3 and transglutaminase activity are all required for the translocation of factor XIII-A from the activated platelet membrane into the fibrin network
　Joanne L Mitchell, Nicola J Mutch
OS4-03--Plasminogen activator inhibitor type 1 in platelets induces thrombogenicity by increasing thrombolysis resistance under shear stress
　Kazuya Hosokawa, Tomoko Onishi, Hisayo Sameshima, Tomoka Nagasato, Tetsumei Urano
OS4-04 (PS2-10)--Optimising the degradation of NETs to destabilise fibrin clot structure and promote fibrinolysis
　Matthew Locke, Robert Francis, Colin Longstaff
OS4-05--Polyphosphate facilitates uPA-mediated fibrinolysis
　Claire S. Whyte, Nicola J. Mutch
OS4-06--Effects of IL-33 on tissue factor expression in human monocyte subsets
　Stefan Stojkovic, Asa Thulin, Lena Hell, Sabine Rauscher, Johanna Baumgartner, Marion Gröger, Cihan Ay, Johann Wojta, Agneta Siegbahn, Mikael Aberg

Oral Session 5---Tissue Remodeling 1

10:45 am- Wednesday, Oct 19th
　Chairs: Hua-Lin Wu and Taiichiro Seki
OS5-01--Role of α2-antiplasmin in bone loss induced by ovariectomy in mice
　Naoyuki Kawao, Akihito Shiomi, Kiyotaka Okada, Osamu Matsuo, Masao Akagi, Hiroshi Kaji
OS5-02--Epithelial thrombomodulin enhances plasminogen-induced vascular endothelial growth factor expression in wound healing
　Hua-Lin Wu, Po-Ku Chen, Guey-Yueh Shi
OS5-03--Inhibition of fibrinolysis delays the liver regeneration through the suppression of cell cycle progression
　Atsushi Miura, Kouta Koizumi, Takashi Hosono, Taiichiro Seki
OS5-04--Fibrin is dispensable and fibrinolysis is essential for fracture repair and prevention of heterotopic ossification
　Masato Yuasa, Nicholas A. Mignemi, Herbert S. Schwartz, Atsushi Okawa, Toshitaka Yoshii, Matthew J. Flick, Jay L. Degen, Jonathan G. Schoenecker
OS5-05--Proinflammatory cytokines and matrix metalloproteinases produced by adventitial adipocytes promotes abdominal aortic aneurysm development
　Hiroki Tanaka, Yuko Suzuki, Hideto Sano, Naoki Unno, Mitsutoshi Setou, Tetsumei Urano
OS5-06 (PS4-02)--Tissue Plasminogen Activator promotes the formation of intracerebral aneurisms
　Romain Goulay, Paul-Emile Labeyrie, Sara De Lizarondo Martinez, Eric Maubert, Cyrille Orset, Denis Vivien

Oral Session 6---Trauma and Bleeding

2:30 pm- Wednesday, Oct 19th
　Chairs: Koji Yamamoto and Hideo Wada
OS6-01--Current monitoring of fibrinolytic activation in critically ill patients: point-of-care testing (ROTEM)
　Vladimir A. Sukhanov, Olga Y. Ptrova, Alexander L. Levit
OS6-02--Systemic Coagulopathy and Acute Lung Injury is Initiated by Traumatic Brain Injury
　Hideki Yasui, Deborah L. Donahue, Mark Walsh, Francis J. Castellino, Victoria A. Ploplis
OS6-03--Early administration with fibrinogen concentrate or cryoprecipitate contributes to the better prognosis for survival of severe traumatic patients
　Koji Yamamoto, Atsushi Yamaguchi, Koichi Inokuchi, Makoto Sawano, Satoru Sugiyama, Kazuhiro Sugiyama, Shigeko Nishimura, Hiroshi Fujita
OS6-04--Clinical and pathophysiologic differences between Traumatic Induced Coagulopathy (TIC) and Disseminated Intravascular Coagulation (DIC)
　Mark Walsh, Francis J. Castellino, Victoria A. Ploplis, Scott Thomas, Zach Crowell, Daniel Hake, Sarah Greve, Deborah Donahue, Anton Crepinsek, Cheney McWilliams

Oral Session 7---The Cutting Edge Research

3:30 pm- Wednesday, Oct 19th
OS7-01--Novel insights into the role of the tPA/plasmin system in the amygdala
　Robert Pawlak, Mariusz Mucha, Alberto Labrador-Ramos, Jaison Kolenchery, Malgorzata Bajor, Benjamin Attwood, Anna Skrzypiec, Valentina Brambilla, Michal Swiatek, Barbara Pijet, Yusuke Sakai, Nobuo Nagai, Leszek Kaczmarek, Ryszard Przewlocki
OS7-02--Tissue plasminogen activator (tPA) and platelet-derived growth factor-C (PDGF-CC) a promising common pathway for therapeutic interventions in the CNS
　Daniel A. Lawrence, Linda Fredriksson, Sebastian A. Lewandowski, Enming J. Su, Ingrid Nilsson, Robert L. Medcalf, U. Eriksson

Oral Session 8---Plasminogen Activation System and Neuroscience

10:30 am- Thursday, Oct 20th
　Chairs: Robert Pawlak and Nobuo Nagai
OS8-01--Key role of vascular tissue-type plasminogen activator in neurovascular coupling
　Antoine Anfray, Denis Vivien, Cyrille Orset
OS8-02 (PS3-06)--The type-4 desintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-4): a new actor in the central nervous system myelination processes
　Mathilde Pruvost, Matthieu Lepine, Camille Leonetti, Eric Maubert, Evelyne Emery, Fabian Docagne, Denis Vivien
OS8-03 (PS4-01)--Comprehensive Modeling for Capillary Morphometry and Blood Flow in Wildtype and tPA-/- Mice
　Randy C. Stevenson, Tamara K. Stevenson, Linda Fredriksson, Daniel A. Lawrence
OS8-04--Neuronal trafficking of the tPA
　Sophie Lenoir, Yannick Hommet, Laurent Lebouvier, Denis Vivien
OS8-05--Astrocytes at the crossroad of plasminogen activation and plasmin clearance in the brain
　Fabian Docagne, Aurélien Briens, Isabelle Bardou, Frédéric Cassé, Lindsey A. Miles, Robert J. Parmer, Denis Vivien
OS8-06--BAC Technology to Elucidate the Localization and Function of Tissue Plasminogen Activator in the Brain
　Tamara K. Stevenson, Carina Castellanos, Kris Mann, Mark Warnock, Enming Su, Geoff G. Murphy, Daniel A. Lawrence

Oral Session 9---Infection, Sepsis, DIC

9:00 am- Friday, Oct 21st
　Chairs: Shaun Lee and Ruby Law
OS9-01--The plasminogen activator protease of Yersinia pestis overcomes fibrin(ogen)-mediated host defenses to cause pneumonic plague
　Daniel L. Zimbler, Jay A. Schroeder, Justin L. Eddy, Lindsay M. Gielda, Jr-Shiuan Lin, Jay L. Degen, Wyndham W. Lathem
OS9-02--Role of the Plasminogen-binding Group A Streptococcal M Protein (PAM) in Bacterial Virulence: Gene Regulation and Protein Translocation
　Jeffery A. Mayfield, Zhong Liang, Victoria A. Ploplis, Francis J. Castellino
OS9-03 (PS3-02)--Evidence That Matrix Metalloproteinase-9 Mediates the Harmful Effects of Alpha2-Antiplasmin in Ischemic Stroke
　Satish Singh, Aiilyan K. Houng, Guy L. Reed
OS9-04--Intravenous thrombolysis generates large amounts of bradykinin in stroke patients: new therapeutic target?
　Sara Martinez de Lizarrondo, Oscar Marcos-Contreras, Denis Vivien, Maxime Gauberti

Oral Session 10---Tissue Remodeling 2

11:00 am- Friday, Oct 21st
　Chairs: Takayuki Iwaki and Malyszko Jolanta
OS10-01--A Plasminogen Deficiency Attenuates Atherosclerosis in a murine model of typeⅡa familial hypercholesterolemia
　Chiharu Miyajima, Takayuki Iwaki, Victoria A Ploplis, Kazuo Umemura, Francis J Castellino
OS10-02--ADAMTS13 deficiency promotes hepatic microthrombosis in a murine model of diet-induced obesity
　Roger Lijnen, Lotte Geys, Elien Roose, Karen Vanhoorelbeke
OS10-03--Roles of alpha2-antiplasmin on pathogensis of non-alcoholic steatohepatitis under high-fat diet feeding
　Nobuo Nagai, Miyabi Ono, Takashi Utsuno, Takahiro Hirata
OS10-04--Anti-Inflammatory Effect of Protease-Activated Receptor-1 Agonists on the Cell Line RBL-2H3 at Hyperglycemia
　Liubov Gorbacheva, Irina Babkina, Ivan Golyako, Svetlana Strukova
OS10-05--Zonulin, YKL-40, inflammation in kidney transplant recipients
　Jacek Malyszko
OS10-06--Endocan and Vascular adhesion protein-1 is significantly elevated in heart transplant recipients and chronić heart failure patients with comorbidities
　Jolanta Malyszko, Piotr Przybylowski, Slawomir Dobrzycki, Hanna Bachorzewska-Gajewska, Jacek S. Malyszko

Oral Session 11---Thrombolysis

2:30 pm- Friday, Oct 21st
　Chairs: Guy L Reed and Teruto Hashiguchi
OS11-01 (PS5-03)--Evaluation of the in vivo action of SMTP-7, a novel small-molecule anti- inflammatory thrombolytic for ischemic stroke
　Eriko Suzuki, Yudai Kunikiyo, Naoki Matsumoto, Hironobu Sawada, Naoko Nishimura, Keiko Hasegawa, Keiji Hasumi
OS11-02-- Nonclinical development and the first-in-human phase I study of TMS-007, a member of the anti-inflammatory small-molecule thrombolytic SMTP
　Keiji Hasumi, Naoko Nishimura, Keiko Hasegawa, Hironobu Sawada, Eriko Suzuki, Kazuo Handa, Teiji Tominaga, Kuniyasu Niizuma, Yurie Wakabayashi, Takashi Moritoyo, Tsutomu Yamazaki, P1 Unit Group Tokyo Univ
OS11-03--Development of Venous Thrombosis Requires Suppression of Endogenous Fibrinolysis
　Satish Singh, Aiilyan K. Houng, Guy L. Reed
OS11-04--Patients with FXI deficiency display a hitherto unknown resistance to TAFIa. Correlation with bleeding phenotype
　Francesca Incampo, Antonio Cannavò, Marzia Menegatti, Simona M. Siboni, Federic Zaccaria, Nicola Semeraro, Flora Peyvandi, Mario Colucci
OS11-05--Development of online apps to simplify and standardise the analysis of fibrinolysis experimental data
　Colin Longstaff
OS11-06-- Towards a 3D Patient-Specific Computational Model of Thrombolysis
　Andris Piebalgs, Xiao Yun Xu